Invest Ophthalmol Vis Sci. 2015 Aug;56(9):5566-73. doi: 10.1167/iovs.15-16531.

Author information

Department of Ophthalmology Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, People’s Republic of China; Key Laboratory of Myopia, Ministry of Health, People’s Republic of China; and Shanghai Key Laboratory of Visual Impairment and Resto.
Parkway Health Hongqiao Medical Center, Shanghai, People’s Republic of China.



The purpose of this study was to assess the inflammatory status of the aqueous humor in the fellow eye after uneventful cataract surgery in the first eye.


At the screening stage, aqueous humor samples from 15 firsteye and 15 second-eye cataract patients were collected just before cataract surgery and assayed using human cytokine antibody array. Screened cytokines were then verified using a suspension array system with aqueous humor samples obtained from 35 firsteye and 36 second-eye cataract patients.


The cytokine antibody array revealed that interleukin-1 receptor antagonist (Il-1ra) and macrophage inflammatory protein (MIP)-1a and MIP-1b were expressed at high levels in firsteye patients and were lower in second-eye patients, whereas opposite trends were found for monocyte chemoattractant protein 1 (MCP-1) and for regulated on activation, normal T expressed and secreted (RANTES) (all, P < 0.05, Student’s t-test). However, only MCP-1 and IL-1ra were significantly different between the two groups after Bonferroni correction (both P < 0.00125). In the replication stage, the suspension cytokine array revealed that only MCP-1 expression was significantly greater in the aqueous humor of second-eye patients than in that of firsteye patients (P = 0.0067, Student’s t-test).


This study revealed that expression of MCP-1, a pain-related inflammatory chemokine, was significantly increased in aqueous humor in the contralateral eye after firsteye cataract surgery. This suggests there may be a sympathetic ophthalmic type uveitis in the contralateral eye after firsteye cataract surgery and that may help to explain why second-eye phacoemulsification is often more painful. ( number, NCT01824927.)